Induction of endoplasmic reticulum (ER) stress by homocysteine has been reported in a variety of cell types. To test if exposure to homocysteine also leads to ER stress response in the adipocyte, we examined mRNA expressions of C/EBP homologus protein (CHOP), an ER stressmediated protein.
terial was ground (Retsch model ZM100 centrifugal grind ing mill), ovendried at 60 °C for 72 h, and analyzed for total phenolics, C, N, and P as described above.
About 50 mg of seedling material was collected, shockfrozen in liquid nitrogen, and homogenized with a Retsch mill (Verder Scientific). The total RNA was extracted with the Plant RNA Mini Kit (OMEGA BioTek) and treated with DNase I (Thermo Fisher Scientific).
Retsch , Germany). The milling balls were mm ZrO 2 beads. 10, 20, 50 and 150 g of beads were applied and milling was carried out without pearls as a benchmark.
PEERREVIEWED ARTICLE ... oil mill effluent (POME) and the addition of cow manure reduced the mortality rate and ... were ground to sizes of 5 mm to 10 mm using a Glen Creston Beater mill (Retsch , Haan, Germany), soaked for 2 weeks, and drained well before use.
Mature pollen grains were grounded with Mixer Mill (MM 300; Retsch). Homogenization buffer (100 mM potassium chloride, 5 mM DTT, 1 mM EDTA and 50 mM Tris pH ) containing high salt (750 mM sodium chloride) and protease inhibitor cocktail was added.
Frozen roots (c. 100 mg) were ground in liquid N 2 using a Retsch M301 ball mill (Restch, Düsseldorf, Germany) for 3 min and then phenolic compounds were extracted from the tissue with 1 ml of 100% methanol by homogenization in the same mill for 5 min.
Retsch MM 301 mixer mill; 2 x adaptor racks; Marsh D; National Health and Medical Research Council (NHMRC)/Equipment Grants. 2001 The identification of novel genes involved in the intiation and development of thyroid neoplasia ; Marsh D, Robinson B; National Health and Medical Research Council (NHMRC)/Project Grants.
Abstract. Propionibacterium freudenreichii is used as a ripening culture in Swiss cheese manufacture. It grows when cheeses are ripened in a warm room (about 24°C). Cheeses with an acceptable eye formation level are transferred to a cold room (about 4°C), inducing a marked slowdown of propionic fermentation, but P. freudenreichii remains active in the cold.
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The loaded strips were used for isoelectric focusing in an Ettan IPGphore 3 device to an extent of 66,567 Vh and equilibrated twice for 15 min with 6 M urea, 75 mM TrisHCl, pH, % glycerol, and 2% sodium dodecyl sulfate (SDS).
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In the case of liver and LD muscle, samples were previously freezedried for three days in a lyophilizer (Lyoquest, Spain) and ground in a Mixer Mill MM400 (Retsch Technology, Germany) until tissues were completely powdered.
Thermal Properties of Extruded InjectionMolded Poly(lactic acid) and Milkweed Composites: Degradation Kinetics and Enthalpic Relaxation Abdellatif A. Mohamed,1 V. L. Finkenstadt,2 D. E. Palmquist,3 P. RayasDuarte4 1Cereal Products and Food Science Unit, National Center for Agriculture Utilization Research, USDAARS, Peoria, IL 61604
Polyribosomes were pelleted through a sucrose cushion (20 mM HepesKOH pH, 10 mM MgAc, M KAc, M sucrose, 2 mM DTT) and the ribosome pellet resuspended in subunit separation buffer lacking DTT (50 mM HEPESKOH pH, 5 mM MgCl 2, M KCl) to a final concentration of about 50 OD 260 units per mL.
RETSCH is the world's leading manufacturer of laboratory mills jaw crushers, sieve shakers sieves as well as assisting technologies. ... mm mixer mill grinding machine glen creston. ... mi er grinder life; Cuisinart Coffee Maker Problems Grinder Stuck; mining equipment mixer grinder price list; Cement Grinder Plants; About.
Cell pellets or tissues were ground in a Retsch Mixer Mill MM 400 under cryogenic conditions; resuspended in % IGEPAL, 50 mmol/L Tris (pH 8), ... (endoplasmic reticulum aminopeptidase associated with antigen processing) ... Aguilar MI, Croft NP,
The dried biomass was then ground in a Retch MM 301 mixer mill (Retsch , Haan, Germany), and the resulting mycelial powder was vacuumed through a highflow respirablethoracic cyclone ( Cyclone; BGI, Waltham, MA, USA) at liters min −1 onto the polyllysinecoated polycarbonate filters.
and 2,787 kcal/kg of DM (Adeola and Ileleji, 2009) for 3wkold broilers. In the current study, we compared ileal digestible energy (IDE), ME, and MEn of DDG and DDGS for 6wkold broiler chickens.
20mm Stainless Steel Grinding Ball This ball is for use in the MM 200 mixer mill (). Composition is approximately % Fe 13% Cr. Approximate density is 7700kg/m3.
Induction of endoplasmic reticulum (ER) stress by homocysteine has been reported in a variety of cell types. To test if exposure to homocysteine also leads to ER stress response in the adipocyte, we examined mRNA expressions of C/EBP homologus protein (CHOP), an ER stress‐mediated protein.
The tencm stem piece (36–46 cm from the base) that was collected for the chemical analyses was freezedried for about 48–72 h, cut into small pieces (1 cm long × 1 mm diameter) and ground into a rough powder using a centrifugal mill (Retsch ZM 200, Haan, Germany).
To each tube 750 μl of zirconia/silica beads ( mm) (BioSpec, number z, Bartlesville, OK, USA) were added and beadbeating was performed at 30 cycles/s for 5 minutes, followed by 10 minutes rest, and beadbeating again for 5 minutes (Retsch MM 300 mixer mill, Haan, Germany).
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